cd36 overexpression Search Results


plasmid overexpressing cd36  (Igene Biotechnology Inc)
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Igene Biotechnology Inc plasmid overexpressing cd36
Amelioration of lipid accumulation in PA-induced HepG2 cells by FV. (a) Oil red O was used to measure the level of lipid accumulation (magnification 100x, scale bar = 250 μ m). (b) The oil red O-positive area was analyzed and quantified. (c–j) The relative mRNA expression levels of FABP1 , SCD1 , <t>CD36</t> , HMGCR , ACACA , CCL5 , IL-1β , and TNF-α were determined by qRT-PCR. (k) The proinflammatory factor protein levels of IL-1 β , IL-6, and TNF- α . (l) The protein levels related to lipid metabolism of SREBP-1, ACOX1, CD36, CPT1 α , and HMGCR were analyzed by western blotting, and the relative ratios were calculated and expressed as the mean ± SD; n = 3. # P < 0.05 means that the difference between the NC group and the PA group is significant. ∗ P < 0.05 means that the difference between the PA group and the FV (30 mg/mL) group or the FV (15 mg/mL) group is significant.
Plasmid Overexpressing Cd36, supplied by Igene Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amelioration of lipid accumulation in PA-induced HepG2 cells by FV. (a) Oil red O was used to measure the level of lipid accumulation (magnification 100x, scale bar = 250 μ m). (b) The oil red O-positive area was analyzed and quantified. (c–j) The relative mRNA expression levels of FABP1 , SCD1 , CD36 , HMGCR , ACACA , CCL5 , IL-1β , and TNF-α were determined by qRT-PCR. (k) The proinflammatory factor protein levels of IL-1 β , IL-6, and TNF- α . (l) The protein levels related to lipid metabolism of SREBP-1, ACOX1, CD36, CPT1 α , and HMGCR were analyzed by western blotting, and the relative ratios were calculated and expressed as the mean ± SD; n = 3. # P < 0.05 means that the difference between the NC group and the PA group is significant. ∗ P < 0.05 means that the difference between the PA group and the FV (30 mg/mL) group or the FV (15 mg/mL) group is significant.

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Ficus hirta Vahl. Ameliorates Nonalcoholic Fatty Liver Disease through Regulating Lipid Metabolism and Gut Microbiota

doi: 10.1155/2022/3474723

Figure Lengend Snippet: Amelioration of lipid accumulation in PA-induced HepG2 cells by FV. (a) Oil red O was used to measure the level of lipid accumulation (magnification 100x, scale bar = 250 μ m). (b) The oil red O-positive area was analyzed and quantified. (c–j) The relative mRNA expression levels of FABP1 , SCD1 , CD36 , HMGCR , ACACA , CCL5 , IL-1β , and TNF-α were determined by qRT-PCR. (k) The proinflammatory factor protein levels of IL-1 β , IL-6, and TNF- α . (l) The protein levels related to lipid metabolism of SREBP-1, ACOX1, CD36, CPT1 α , and HMGCR were analyzed by western blotting, and the relative ratios were calculated and expressed as the mean ± SD; n = 3. # P < 0.05 means that the difference between the NC group and the PA group is significant. ∗ P < 0.05 means that the difference between the PA group and the FV (30 mg/mL) group or the FV (15 mg/mL) group is significant.

Article Snippet: The plasmid overexpressing CD36 was designed and built by iGene Biotechnology Co., Ltd. (Guangzhou, China) to generate a CD36-overexpressed (CD36 OE) cell line, and an empty vector was used as a control. siCD36 was synthesized by RIBOBIO Co., Ltd. (Guangzhou, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Effects of FV on liver lipogenesis-related markers in the mice fed with HFD. (a–h) The relative mRNA expression levels of Acaca , Cd 36, Cpt 1 α , Srebp- 1, Hmgcr , Pparα , Pparγ , and Scd 1 were determined by qRT-PCR. (i) The lipid metabolism relevant protein levels of SREBP-1, ACOX1, CD36, CPT1 α , and HMGCR were analyzed by western blotting, and the relative ratios were calculated. (j–l) Serum IL-6, IL-1 β , and TNF- α were identified by ELISA kits. (m, n) The relative mRNA expression levels of IL-1 β , TNF- α , and Ccl5 were identified by qRT-PCR. (p) The proinflammatory factor protein levels of IL-1 β , IL-6, and TNF- α were analyzed by western blotting, and their relative ratios were calculated. The densitometry was obtained by averaging repeated experiments results, normalized to GAPDH. The data are expressed as the mean ± SD, n = 3. # P < 0.05 means there is significant difference between the NFD group and the HFD group. ∗ P < 0.05 means the difference between the HFD group and the FV-L group or the FV-H group is significant.

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Ficus hirta Vahl. Ameliorates Nonalcoholic Fatty Liver Disease through Regulating Lipid Metabolism and Gut Microbiota

doi: 10.1155/2022/3474723

Figure Lengend Snippet: Effects of FV on liver lipogenesis-related markers in the mice fed with HFD. (a–h) The relative mRNA expression levels of Acaca , Cd 36, Cpt 1 α , Srebp- 1, Hmgcr , Pparα , Pparγ , and Scd 1 were determined by qRT-PCR. (i) The lipid metabolism relevant protein levels of SREBP-1, ACOX1, CD36, CPT1 α , and HMGCR were analyzed by western blotting, and the relative ratios were calculated. (j–l) Serum IL-6, IL-1 β , and TNF- α were identified by ELISA kits. (m, n) The relative mRNA expression levels of IL-1 β , TNF- α , and Ccl5 were identified by qRT-PCR. (p) The proinflammatory factor protein levels of IL-1 β , IL-6, and TNF- α were analyzed by western blotting, and their relative ratios were calculated. The densitometry was obtained by averaging repeated experiments results, normalized to GAPDH. The data are expressed as the mean ± SD, n = 3. # P < 0.05 means there is significant difference between the NFD group and the HFD group. ∗ P < 0.05 means the difference between the HFD group and the FV-L group or the FV-H group is significant.

Article Snippet: The plasmid overexpressing CD36 was designed and built by iGene Biotechnology Co., Ltd. (Guangzhou, China) to generate a CD36-overexpressed (CD36 OE) cell line, and an empty vector was used as a control. siCD36 was synthesized by RIBOBIO Co., Ltd. (Guangzhou, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

Alleviation of lipid accumulation and inflammation by FV through regulating CD36 in HepG2 cells with PA inducement. (a, c) CD36 protein levels after CD36 knockdown or CD36 overexpression and quantitative analysis. (b, d) TG contents of HepG2 cells in different treatment groups. (e, f) Oil red O to examine the accumulation level of lipid in HepG2 cells and their quantified scores (magnification 100x, scale bar = 250 μ m). (g) The mRNA levels of SCD1, PPAR γ , ACACA, SREBP-1, IL-1 β , and CCL5 in siCD36 HepG2 cells with or without FV administration under the condition of PA inducement. (h) The mRNA levels of PPAR γ , SREBP-1, IL-1 β , and TNF- α in CD36 OE HepG2 cells with or without FV administration under the condition of PA inducement. (i) The protein levels of CD36, HMGCR, ACOX1, and SREBP-1 in siCD36 HepG2 cells. (j) The protein levels of CD36, HMGCR, ACOX1, and SREBP-1 in CD36 OE HepG2 cells. n = 3, ∗ P < 0.05 vs. NC group for (a, c); # P < 0.05 vs. NC group, ∗ P < 0.05 vs. PA group or PA+siCD36 group/PA+CD36 OE group (b–j).

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Ficus hirta Vahl. Ameliorates Nonalcoholic Fatty Liver Disease through Regulating Lipid Metabolism and Gut Microbiota

doi: 10.1155/2022/3474723

Figure Lengend Snippet: Alleviation of lipid accumulation and inflammation by FV through regulating CD36 in HepG2 cells with PA inducement. (a, c) CD36 protein levels after CD36 knockdown or CD36 overexpression and quantitative analysis. (b, d) TG contents of HepG2 cells in different treatment groups. (e, f) Oil red O to examine the accumulation level of lipid in HepG2 cells and their quantified scores (magnification 100x, scale bar = 250 μ m). (g) The mRNA levels of SCD1, PPAR γ , ACACA, SREBP-1, IL-1 β , and CCL5 in siCD36 HepG2 cells with or without FV administration under the condition of PA inducement. (h) The mRNA levels of PPAR γ , SREBP-1, IL-1 β , and TNF- α in CD36 OE HepG2 cells with or without FV administration under the condition of PA inducement. (i) The protein levels of CD36, HMGCR, ACOX1, and SREBP-1 in siCD36 HepG2 cells. (j) The protein levels of CD36, HMGCR, ACOX1, and SREBP-1 in CD36 OE HepG2 cells. n = 3, ∗ P < 0.05 vs. NC group for (a, c); # P < 0.05 vs. NC group, ∗ P < 0.05 vs. PA group or PA+siCD36 group/PA+CD36 OE group (b–j).

Article Snippet: The plasmid overexpressing CD36 was designed and built by iGene Biotechnology Co., Ltd. (Guangzhou, China) to generate a CD36-overexpressed (CD36 OE) cell line, and an empty vector was used as a control. siCD36 was synthesized by RIBOBIO Co., Ltd. (Guangzhou, China).

Techniques: Knockdown, Over Expression

The speculative mechanism of Ficus hirta Vahl. for treatment of NAFLD in HFD-fed mice. FV alleviates nonalcoholic fatty liver disease through adjusting lipid metabolism and inflammation via downregulation of CD36 and regulating the structure of gut microbiota.

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Ficus hirta Vahl. Ameliorates Nonalcoholic Fatty Liver Disease through Regulating Lipid Metabolism and Gut Microbiota

doi: 10.1155/2022/3474723

Figure Lengend Snippet: The speculative mechanism of Ficus hirta Vahl. for treatment of NAFLD in HFD-fed mice. FV alleviates nonalcoholic fatty liver disease through adjusting lipid metabolism and inflammation via downregulation of CD36 and regulating the structure of gut microbiota.

Article Snippet: The plasmid overexpressing CD36 was designed and built by iGene Biotechnology Co., Ltd. (Guangzhou, China) to generate a CD36-overexpressed (CD36 OE) cell line, and an empty vector was used as a control. siCD36 was synthesized by RIBOBIO Co., Ltd. (Guangzhou, China).

Techniques: